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  • An Experimental Granuloma Model in Rat Spinal Cord

    Authors

    T. Erhan Cosan, MD
    Stephen W. Coons, MD*
    Curtis A. Dickman, MD†
    Sacit G×ulec, MD‡
    Esref Tel, MD

    Departments of Neurosurgery and ‡Anesthesiology and Reanimation, Osmangazi University, Eskisehir, Turkey
    Divisions of †Neurological Surgery and *Neuropathology, Barrow Neurological Institute, St. Joseph’s Hospital and Medical Center, Phoenix, Arizona

    Abstract

    An experimental model is described for producing space-occupying lesions of the spinal cord with kaolin, whose effect on cellular proliferation is well known. Fifteen Sprague-Dawley rats were used in this study. In 10 rats, 0.1 ml (200 mg/ml) kaolin was injected into the posterior subarachnoid space of the thoracic spinal cord at any level between T6 and T10 over 20 seconds. Five control rats were injected with saline in a similar manner. All animals were sacrificed 4 to 5 weeks after the injections, and their spinal cords were harvested for analysis by light microscope. Space-occupying lesions, which appeared to be granulomas, were observed in the posterior spinal cord in five kaolin-injected rats. No gross abnormalities were apparent in the control rats. If a natural space-occupying lesion associated with cellular proliferation in the spinal cord is needed, kaolin-induced granulomas may represent a realistic and reliable experimental model.

    Key Words: experimental model, granuloma, kaolin, spinal cord

    Kaolin, a ceramic fiber that contains mostly hydroxylated aluminum silicate [Al(4)Si(4)O(10) (OH)(8)], is used as an additive compound in the ceramic industry.8 This refractory clay, whose toxic effect is well known, has been introduced into a variety of tissues to produce inflammation, cellular toxicity, and space-occupying lesions, especially granulomas.12,14,15

    In neurological research, kaolin has been used to induce cerebral granulomas, which act as an epileptic foci. In the spinal canal, adhesions produced by kaolin lead to syringomyelia; when instilled in the cisterna magna, it causes hydrocephalus.2-4,7,11,17 Kaolin, however, has not been used in experimental studies to produce intramedullary space-occupying mass lesions.

    table-1 chemical constituents of and their concentrations in kaolinInitially, we proposed to produce spinal syrinx cavities by injecting kaolin into the subarachnoid space. Then incidental intramedullary granulomas that might be suitable for spinal cord research were discovered in some rats. Producing mass lesions for spinal cord research must be simple and mimic natural processes if the results are to be generalizable. Some models of extra- and intramedullary compression of the spinal cord have been far from natural nor have they involved cellular space-occupying lesions.1,5 The present kaolin model, however, is a realistic, reliable, and easy method for producing space-occupying lesions involving the spinal cord.

    Material and Methods

    After the experimental protocol was approved by and received support from TICAM (Medical and Surgical Research Center of Osmangazi University), 15 Sprague-Dawley rats (300-350 gm) were anesthetized intramuscularly with a xylazine hydrochloride (10 mg/kg) and ketamine (60 mg/kg) mixture. A single- or double-level laminectomy was performed between T6 and T10 under the operating microscope. A special frame designed by TICAM technical service, mounted with a 29-gauge needle and a 10 µl Hamilton syringe, was brought into the operating field. In 10 rats the needle was inserted into the subarachnoid space and 0.1 ml kaolin (200 mg/ml) was injected over 20 seconds. Five control rats had 0.1 ml normal saline injected into the subarachnoid space in the same fashion.

    All animals were sacrificed 4 to 5 weeks after injection by transcranial perfusion of 2.5% glutaraldehyde and 2% paraformaldehyde in 0.2 mol/L phosphate buffer (pH 7.4), and the spinal cords from T6 to T10 were quickly removed. Light microscopy specimens were fixed in 2.5% glutaraldehyde, dehydrated in ethanol, and embedded in paraffin. Histological analysis was performed on sections stained with hematoxylin and eosin.

    A radiographic fluorescent assay (WRF-8680 ± 266; ARL, Swiss) of the chemical composition of the kaolin (Table 1) showed that approximately 88% (of a total 91.15%) of the compounds in the kaolin were aluminum and silicate.

    Figure 1. Granuloma replaces the posterior column on the right side of the spinal cord

    Figure 1. Granuloma replaces the posterior column on the right side of the spinal cord (hematoxylin and eosin, original magnification x20).

    Results

    After the injection, two rats in the kaolin-injected group exhibited weakness of the lower extremities that recovered within 24 to 48 hours. After 48 hours and until they were sacrificed, no animal exhibited a neurological abnormality associated with running, standing, jumping, or responding to painful stimuli. No rat in the control group exhibited a gross abnormality of their spinal cord. Space-occupying lesions were observed in the posterior spinal cord adjacent to the injected area in five rats who had kaolin injected into the posterior subarachnoid space. These gross intraparenchymal lesions were especially close to the injected subarachnoid region.

    Figure 2. The granuloma is composed of spindle cells and epithelioid histiocytes.

    Figure 2. The granuloma is composed of spindle cells and epithelioid histiocytes. No multinucleate histiocytes are present. The lesion is expansive and entraps no spinal cord parenchyma (hematoxylin and eosin, original magnification x100).

    In the posterior portion of the spinal cord, kaolin crystals were traced intraparenchymally. In all rats in the kaolin group, the subarachnoid space was completely obstructed and arachnoidal adhesions were present. Well-formed granulomas were present in the posterior column of five spinal cords (Fig. 1). One of these specimens also had a similar granuloma in the subarachnoid space. The granulomas were primarily composed of spindle-shaped or epithelioid histiocytes and some lymphocytes. Multinucleate histiocytes were not present (Fig. 2). Crystalline foreign material was dispersed throughout the granulomas (Fig. 3). Rather than being infiltrative, the space-occupying lesions were well-demarcated and expansive.

    Figure 3. Fragments of crystalline foreign material (arrows) within the granuloma

    Figure 3. Fragments of crystalline foreign material (arrows) within the granuloma (hematoxylin and eosin, original magnification x400).

    Discussion

    Various experimental models 1,6,9,10 for spinal cord compression have been described: inserting implantable artificial masses, suspending tumor cells, and advancing screw or balloon catheters into the spinal canal. Inserting tumor cells into the spinal canal leads to aggressive cellular proliferation that devastates the spinal cord and may kill the animal. Advancing a screw or a balloon catheter into the spinal canal is an unnatural process that fails to mimic a space-occupying lesion of the spinal cord. These methods are extremely different from those associated with space-occupying lesions that occur naturally.

    Kaolin has long been introduced into the spinal canal and spinal cord. Although it is known to produce granulomatous inflammation in the spinal canal, its use to produce space-occupying lesions in the spinal cord has not been reported.3,4,11,13 Romero et al.13 have reported that granulomatous leptomeningomyelitis was accompanied by an intense glial reaction after kaolin was injected subdurally in cats. They evaluated the light and electron microscopic aspects of meningeal granulomatous and spinal cord glial reactions but found no evidence of macroscopic spinal cord granulomas. In our study, introducing kaolin into the subarachnoid space led to the formation of intramedullary granulomas adjacent to the injected area in five rats. In earlier experimental models, the doses of the kaolin introduced into the spinal canal were lower than those used in our rat model.3,4,11 The granulomas might therefore have developed because high doses of kaolin (0.1 ml, 200 mg/ml), which are excessive for the small cross-sectional area of a rat’s spinal canal, were used. Higher concentrations of kaolin would likely produce granulomas even more reliably. The kaolin introduced into the subarachnoid space was also traced spontaneously into medullary parenchyma, which then caused cellular proliferation. Mononuclear cellular proliferation in response to intramedullary kaolin produced discrete granulomas in the posterior column. The space-occupying lesions were expansive, compressing rather than infiltrating the surrounding spinal cord parenchyma.

    Although the number of rats used in this study was small, the results represent the first indication that spinal cord granulomas that might serve as an acceptable model for a naturally occurring medullary mass can be produced experimentally in an animal. Kaolin could be introduced into various subarachnoid regions of the spinal canal to produce different neurological deficits. With granulomas at various locations in the spinal cord, motor or sensory evoked potentials could be measured to reflect function. In our kaolin-injected group, the abilities to stand and jump were unimpaired on the day of sacrifice. Evaluating the sensory status of rats by clinical observation is fairly difficult. The somatosensory evoked potentials of animals with posterior spinal cord granulomas, however, could be measured as an indication of their somatosensory function.

    The spinal cord mass lesions might have formed due to the effect of the various substances in the kaolin, which was primarily composed of aluminum and silicate. Other than the aluminum and silicate, only two other compounds in the kaolin could induce cellular proliferation and inflammation: the titanium dioxide (TiO2) and iron particles.15,16 The concentration of these compounds with kaolin, however, was very low for inducing cellular proliferation and inflammation. Still, these particles might be involved in the formation of the granulomas.

    The present kaolin-induced rat model is simple, inexpensive, and easily reproducible with readily available materials. Intramedullary granulomas were produced adjacent to kaolin-injected regions of the subarachnoid space. Hence, such granulomas could likely be produced in any desired region of the spinal cord. The model offers researchers the potential to evaluate any region of the spinal cord and closely mimics the natural formation of intramedullary tumor lesions. It may be useful for further studies of the pathogenesis of space-occupying lesions of the spinal cord. If a natural space-occupying lesion is desired, spinal cord granulomas can be produced by injecting high doses of kaolin.

    References

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