Flow Cytometry & Genomics Core Facility
Focus and Mission
The Barrow Flow Cytometry and Genomics Core Facility provides support services to support researchers within the Barrow Neurological Institute and worldwide. Included in these services is assistance with experimental design, reagent selection, and data analysis. Please get in touch with us for more information.
Services
Flow Cytometry
- Fluorescence Activated Cell Sorting (FACS): BD FACSAria IIu Cell Sorter with Violet (405 nm), Blue (488 nm), and Red (633 nm) lasers.
- BSL2+ system: Sort live, human tissue
- 9-color detection plus 2 scatter parameters
- Sort collection tube options: 15 mL Falcon tubes, 1.5 mL Eppendorf tubes, well plates (6, 24, 48, 96, and 384), or petri plates
- Controlled sorting temperature
- BSL2+ system: Sort live, human tissue (samples with risk or known infection by certain pathogens is restricted, contact us to request a list of restricted specimens)
- Analytical Flow: BD LSRFortessa X-20 Cell Analyzer with Violet (405 nm), Blue (488 nm), and Red (640 nm) lasers.
- 14-color detection plus two scatter parameters
- Use FluoroFinder to build your own panels:
- Select CYTOMETER:
- St. Josephs Hospital and Medical Center
- Barrow Neurological Institute
- FACS Aria II (OR) LSRFortessa X-20
- Select CYTOMETER:
Genomics
We offer library construction and short-read sequencing of prepared libraries on the Illumina NextSeq2000, capturing a range of 400 million to 1.2 billion reads. Our services include short-read bulk (Takara SMART-Seq) and single-cell RNA (10x) sequencing to capture the transcriptome of human and mouse samples, and we are open to exploring alternative sequencing applications. Please contact us if you are interested.
In addition to library construction and sequencing, we offer various methods of measuring and analyzing genomic materials utilizing our Qubit 4 Fluorometer, QuantStudio 6 Flex, Nanodrop One, QIAxcel Connect, and Nanostring nCounter SPRINT profiler.
Sample Submission Hours
Monday – Friday
8:30 AM – 3:30 PM
(excluding major holidays)
Sample Processing Fees
Flow Cytometry
| Service | Details | In-Network Cost | external cost |
|---|---|---|---|
| FACS – Sort & Setup | FACSAria IIu | $135/hour | $235/hour |
| Analytical Flow | LSRFortessa X-20 | $99/hour | $175/hour |
| External Staining | 10 samples + 3 controls | $99/hour | $175/hour |
| External + Internal Staining | 10 samples + 3 controls | $265/hour | $468/hour |
Genomics
| Service | library type | in-network cost* | external cost* |
|---|---|---|---|
| Whole Transcriptome – Sample Prep & Sequencing | Bulk RNA | $175/sample | $310/sample |
| Whole Transcriptome – Sample Prep & Sequencing | Single Cell RNA | $2740/sample | $4855/sample |
| Library Quant & QC | Any | $70/sample | $120/sample |
| Demultiplexing & FASTQ Generation | Any | $55/run | $100/run |
| RNA-Seq Standard Analysis | Bulk RNA | $100/sample | $195/sample |
| RNA-Seq Standard Analysis | Single-Cell RNA | $200/sample | $390/sample |
Please remember to do the following when publishing any work done with support from the core:
- Acknowledge support from Barrow Neurological Foundation
- Include the Flow Cytometry and Genomics Core Facility RRID#: SCR_021133
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